Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System

<div>SDS-PAGE and western-blotting analysis of insoluble and soluble recombinant SnRK2.1. The reaction was performed in a volume of 50 μL and the soluble fraction and insoluble pellet were separated by centrifugation. 5 μL of the precipitate (P) and 5 μL of the supernatant (S) were analyzed by SDS-PAGE (a), while 1 μL of the precipitate and 1 μL of the supernatant were analyzed by western-blotting (b). The arrow marks indicate the molecular mass of the recombinant SnRK2.1. M, maker; NC-P, precipitate of negative control without plasmid; SnRK2.1-P, precipitate of recombinant SnRK2.1; NC-S, supernatant of negative control without plasmid; SnRK2.1-S, supernatant of recombinant SnRK2.1.</div>

BioMed Research International

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Figure 2

Figure 2: Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System

Figure 2 | Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System 