Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of<i> Arabidopsis thaliana</i> in<i> E. coli</i>-Based Cell-Free System

<div>Purification of the insoluble recombinant SnRK2.1 expressed in cell-free system. Insoluble SnRK2.1 was resuspended with column buffer B containing 8 M urea and then purified using Ni-NTA column. M, maker; Lane 1, resuspension with 8 M urea; Lane 2, flow through; Lane 3, washing with 20 mM imidazole; Lane 4, elution with 300 mM imidazole; Lane 5, pellet could not be dissolved by urea.</div>

BioMed Research International

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Figure 5

Figure 5: Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of<i> Arabidopsis thaliana</i> in<i> E. coli</i>-Based Cell-Free System 

Figure 5 | Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System 