Research Article

Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System

Figure 6

Autophosphorylation kinetics of recombinant SnRK2.1. The autophosphorylation of recombinant SnRK2.1 purified from soluble (closed circles) and insoluble (closed squares) fractions were assayed separately. Closed triangles indicate the blank control. Error bars represent the standard deviations calculated from three individual measurements. The inset is ADP standard curve performed as instruction of the EnzyChrom Kinase Assay Kit.