Research Article
Study of Valproic Acid-Enhanced Hepatocyte Steatosis
Table 1
Sequences of primer pairs used in study.
| | Gene | Forward/reverse primer sequences (5′ to 3′) | Reference |
| | 18S rRNA | CGGACAGGATTGACAGATTG
CAAATCGCTCCACCAACTAA | NT039649 |
| | Cpt1a | CTGAGCCATGAAGCCCTCAA
CACACCCACCACCACGATAA | NM013495 |
| | Acca | GGAGCTAAACCAGCACTCCC
GGCCAAACCATCCTGTAAGC | NM133360 |
| | Fasn | TAGAGCAGGACAAGCCCAAG
GAGGCGTAGTAGACAGTGCAGAG | NM007988 |
| | Scd1 | GGCCTGTACGGGATCATACTG
CAGAGCGCTGGTCATGTAGTA | NM009127 |
| | Acsl1 | AGCACCGTACACTGGAGGAA
AGGAAAACCTCTGGTCCACTG | NM007981 |
| | Gpat | ATGAAACGCACACAAGGCAC
CCCTTATGGACGTCTCGCTC | NM008149 |
| | Dgat1 | TTGACCTCAGCCTTCTTC
CATTGCCATAGTTCCCTTG | NM010046 |
| | Dgat2 | GCTGGCATTTGACTGGAACA
GCCACACGGCCCAGTTT | NM026384 |
| | Plin2 | GAACAGTGGAGTAGATAATG
TGAGAGCCTGGTGATAAG | NM007408 |
| | Cd36 | TTACACATACAGAGTTCGTTATC
TCCAACAGACAGTGAAGG | NM001159558 |
| | Lrp | GACCGACTGGCGAACAAAT
CTGGGTGTTGGTCCTCTGTA | Degrace et al. [21] |
| | Ldlr | CTGTGGGCTCCATAGGCTATCT
GCGGTCCAGGGTCATCTTC | Lelliott et al. [22] |
| | Mttp | ATTGAGCGGTCTGGATTTACAAC
AGGTAGTGACAGATGTGGCTTTTG | Lelliott et al. [22] |
| | Pparg | CGGGCTGAGGAGAAGTCACA
GTCTGTCACACAGTCCTGTCA | NM001127330 |
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