miR-20b Inhibits T Cell Proliferation and Activation via NFAT Signaling Pathway in Thymoma-Associated Myasthenia Gravis

Figure 4

NFAT5 and CAMTA1 are targets of miR-20b. (a) Schematic diagram of putative binding sites of miR-20b in the 3′-UTR of NFAT5. (b) HEK293T cells were cotransfected with miR-20b and NFAT5 luciferase reporter vector. Luciferase activity was analyzed 48 h after transfection. (c) Protein levels of NFAT5 as determined by Western blot analysis in Jurkat cells transfected with miR-20b, anti-miR-20b, or their respective controls (miR-NC or anti-miR-NC). (d) Schematic diagram of putative binding sites of miR-20b in the 3′-UTR of CAMTA1. (e) HEK293T cells were cotransfected with miR-20b and CAMTA1 luciferase reporter vector. Luciferase activity was analyzed 48 h after transfection. (f) Protein levels of CAMTA1 as determined by Western blot analysis in Jurkat cells transfected with miR-20b, anti-miR-20b, or their respective controls (miR-NC or anti-miR-NC). .

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