First Observation of Leptospira interrogans in the Lungs of Rattus norvegicus

Table 1

Results of various laboratory tests performed on three Rattus norvegicus individuals naturally infected in a city park in Lyon (France).

Rat	Weight (g)	Size (mm)	Sex	Age	MAT (serovar)	Organs	Leptospira isolation	Real-time PCR (Ct)	Immunohistochemistry	Warthin-Starry staining
DTO14	206	235	Male	Adult	Copenhageni: 1 : 60; IH; Cynopteri; Mangus: 1 : 120	Kidney; Lung	+; n/a	+ (19, 88); + (34, 15)	+^1; +^2	+^1; +^2
DTO15	239	243	Male	Adult	Copenhageni: 1 : 60; IH; Cynopteri: 1 : 120	Kidney; Lung	−; n/a	−; −	−; −	−; −
DTO16	176	225	Male	Adult	Copenhageni: 1 : 30; IH: 1 : 120	Kidney; Lung	+; n/a	+ (23, 95); −	+^1; +^2	+^1; +^2

IH: Icterohaemorrhagiae serovar; +: presence of leptospires or Leptospira DNA; −: absence of leptospires or Leptospira DNA.
¹The leptospires visualized on the epithelium of the renal tubules.
²The leptospires visualized on the ciliated epithelium of the bronchi.