Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation

Figure 1

Tear protein profile. Equal protein amounts (20 µg/lane/sample) were subjected to electrophoretic separation (SDS-PAGE) and membranes were stained with Ponceau S before image acquisition (see M&M). Note the presence of albumin (60 kDa), low/high IgG bands (40/100 kDa), and fibronectin (200 kDa) in some tear samples (1–5, VKC; 6–8 healthy controls). To retrieve low-expressed antigens, samples showing high albumin/IgGs were treated with a specific preclearing kit (see M&M).