Quiescent and Active Tear Protein Profiles to Predict Vernal Keratoconjunctivitis Reactivation

Figure 3

Plot graphs of the tear expression profile in active and quiescent VKC. Fold changes (Log₂ (FC); -axis) are ranked in Volcano plots according to the statistical significance ( values as negative Log₁₀; -axis). For each marker, FC between case and control values were calculated from mean of Fluorescent Intensity values provided by the software, as described in M&M. The two-sided unpaired -test comparisons of active (a) and quiescent (b) samples were carried out versus controls. Both ±2 FC and were used as initial cut-offs. Red lines indicate differences of ±1 FC (log₂) and blue line shows the initial significance level. Those candidates, having ≥2 FC and initial cut-offs, are localized in the upper left quadrant.

(a)

(b)