Growth profile and protein expression of the recombinant strains harbouring codon-optimized and wild-type UGT72B14 gene. (a) Cell growth of recombinant strains. Data are presented as mean of three biological replicates, and error bar represents the standard deviation (SD). (b) SDS-PAGE analysis of protein expression. Sample of 20 μL was used for SDS-PAGE analysis. UGT72B14-opt = codon-optimized UGT72B14; UGT72B14-wt = wild-type UGT72B14. Lane M: protein molecular weight markers. Lanes 1–3: protein expression at 4, 6, and 9 h after induction. Target protein is indicated by arrows.