Expression of Codon-Optimized Plant Glycosyltransferase UGT72B14 in Escherichia coli Enhances Salidroside Production

Figure 2

Growth profile and protein expression of the recombinant strains harbouring codon-optimized and wild-type UGT72B14 gene. (a) Cell growth of recombinant strains. Data are presented as mean of three biological replicates, and error bar represents the standard deviation (SD). (b) SDS-PAGE analysis of protein expression. Sample of 20 μL was used for SDS-PAGE analysis. UGT72B14-opt = codon-optimized UGT72B14; UGT72B14-wt = wild-type UGT72B14. Lane M: protein molecular weight markers. Lanes 1–3: protein expression at 4, 6, and 9 h after induction. Target protein is indicated by arrows.

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