Functional Roles of Pattern Recognition Receptors That Recognize Virus Nucleic Acids in Human Adipose-Derived Mesenchymal Stem Cells

Figure 3

Involvement of TLR3 and RIG-I in poly(I:C)-triggered immune responses. (a) Knockdown of TLR3 or RIG-I at the mRNA levels. hAD-MSCs were transfected with individual siRNAs targeting a scrambled sequence (SiCtrl), TLR3 (siTLR3), and RIG-I (siRIG-I) and the mRNA levels were determined using real-time PCR. (b) Knockdown of TLR3 or RIG-I at the protein levels. hAD-MSCs were transfected with siCtrl, siTLR3, and siRIG-I. After 24 h, the expression of TLR3 and RIG-I was detected by Western blot. (c) IFN-α and IFN-β secretion. hAD-MSCs were transfected with each siRNA. After 24 h, the cells were stimulated with poly(I:C) and the cytokine levels of IFN-α and IFN-β in media were measured using ELISA. (d) Expression of antiviral proteins. hAD-MSCs were treated as (c). Antiviral protein levels were determined using Western blot. Western blot images represent at least three experiments. Data are the means ± SEM of three experiments. ; .

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