Structural Study of Heterogeneous Biological Samples by Cryoelectron Microscopy and Image Processing

Figure 6

Eigenimages-Substrate Binding. (a) GroEL bound to the substrate rhodanese with the raw images (top) and eigenimages (bottom). Eigenimage 5, highlighted with a yellow box indicates heterogeneity in the trans-ring which is related to the binding of rhodanese (adapted from [5]). (b) Three of the 12 orientation classes (column 1) from GroEL-rhodanese complex after MSA based on the eigenimages, the first six of which are shown in (a). The eigenimages of these classes are shown in columns 2–5 and the heterogeneity in the trans-ring is highlighted with a yellow box (from [5]).

(a)

(b)