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BioMed Research International
Volume 2017 (2017), Article ID 1054129, 10 pages
https://doi.org/10.1155/2017/1054129
Research Article

Correlation between Tissue Characterization and Dynamic Expression of Matrix Metalloproteinase-2 and Its Tissue Inhibitor in Conjunctival Filtering Bleb of Rats

1Department of Ophthalmology, Affiliated Hospital of Qingdao University, Qingdao, China
2Department of Ophthalmology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan, China
3Central Laboratory, Affiliated Hospital of Qingdao University, Qingdao, China

Correspondence should be addressed to Ling Wang

Received 27 March 2017; Revised 2 July 2017; Accepted 6 August 2017; Published 18 September 2017

Academic Editor: Ilias Georgalas

Copyright © 2017 Ling Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. Using rat conjunctival bleb model, we correlated changes morphology and histology in the bleb with changes in MMP-2 and TIMP-2 levels. Methods. Filtering surgeries were performed on rats. Dynamic changes in morphology and histopathology were observed using HE staining. Expression of MMP-2 and TIMP-2 was determined by immunofluorescence microscopy and western blotting. Results. Well-elevated filtering blebs formed and persisted for an average of 12 days. Histological examination showed that inflammatory was dominant in postoperative days 1–3, and proliferating manifestation became the main sign 5 days later. Western blot showed that MMP-2 was downregulated 1 day after surgery, upregulated at 3 days, and observed with a peak at 7 days; then it persisted until 28 days. The difference was statistically significant (F = 280.18, ).TIMP-2 was upregulated 1 day after surgery and observed with a peak at 5 days; then it persisted until 28 days. The difference was statistically significant (F = 145.34, ). Conclusions. During the processes of conjunctival filtering bleb and scar formation in rats, the changes in MMP-2 and TIMP-2 levels in the filtering area, together with a corresponding proliferation of fibroblasts and the accumulation of collagen fibres, resulted in scarring of filtering blebs.