Association between Genetic Polymorphism and Risk of von Willebrand Disease in Pakistan

Table 2

Sequence and specification of primers.

Primer name	Primer sequence	Basic TM	Position	Length	Product
Allele specific
rs 61748511
Normal primer	5′ACTTGACAGGCAGGTGCACT3′	53.8°C	Reverse	20	213 bp
Mutated primer	5′ACTTGACAGGCAGGTGCACC3′	55.9°C	Reverse	20
Counter primer	5′ATTGGTGACGCCCATAGTCC3′	53.8°C	Forward	20
Allele specific
rs 61750595
Normal primer	5′AGGACTTTGAGACGCTCCCCC3′	58.3°C	Forward	21	459 bp
Mutated primer	5′AGGACTTTGAGACGCTCCCCT3′	56.3°C	Forward	21
Counter primer	5′AAGGCAGAGGGTGGAATTGG3′	53.8°C	Reverse	20
Allele specific
rs 61751296
Normal primer	5′AAAGACCTCCTCCTTCACTCC3′	54.4°C	Reverse	21	361 bp
Mutated primer	5′AAAGACCTCCTCCTTCACTCT3′	52.4°C	Reverse	21
Counter primer	5′GCTCAGCTCAAACCAATGCT3′	53.0°C	Forward	20