Identification of Three Novel Splicing Variants and Expression Analysis of Chicken GPR1 Gene

Figure 1

Gel electrophoresis images of GPR1 RACE-PCR products. PCR products were amplified with nested PCR. Gel pictures analysis suggesting the presence of multiple GPR1 variants. Note a single band in 5′-RACE-PCR and multiple amplicons in 3′-RACE-PCR. The PCR products of GPR1 were separated on 1% agarose gel following electrophoresis and visualized with ethidium bromide. “MTs” represents mixtures of cDNA (hypothalamus, pituitary, oviduct, adipose tissues, and muscle tissues). Round 1, Round 2, and Round 3 represent the first-round PCR, the second-round PCR, and the third-round PCR, respectively.