Identification of Three Novel Splicing Variants and Expression Analysis of Chicken GPR1 Gene

Table 1

Primers used to study GPR1.

S. number	Primer name	Sequence (5′- to 3′-end)	Source	Purpose
1.1	GR1-5′-1	CAGATGACAATGGCATTGCCTGGGACTC	Conserved domains of published sequences (XM_015289515.1, 971405019)	To obtain 5′-UTR and; 3′-UTR
1.2	GR1-5′-2	TATGGGCAATGCTGAGGGTGGACTGG
1.3	GR1-5′-3	CTTCCTCAGGCAGGTCATAGAAATAAGAGTAG
2.1	UPM-long	ATAGGGCAAGCAGTGGTATCAACGCAGAGT
	UPM-short	ATAGGGC
3.1	GR1-3′-1	GAGGAAGGCAGTAGCCAAGTGAAGCAGC
3.2	GR1-3′-2	CACACTGAAGTCACTCTGAACTCTACAGATCACT
3.3	GR1-3′-3	GCTACAGAGAGCACATCCCTGACTTACAGTGT
4.1	GPR1-va-F	TGACTGTTCATCACCATCCATCT		To measure expression levels of GPR1-va and GPR1-vb by qRT-PCR, respectively
4.2	GPR1-va-R	ATGCTGCAACCGCACCAC
5.1	GPR1-vb-F	TGTCACCATTTGCTACAAAATAGC
5.2	GPR1-vb-R	ATGCTGCAACCGCACCAC
6.1	β-Actin-F2	TGTGCTGTCCCTGTATGCCTC	Specific regions of Gallus gallusβ-actin (L08165.1); Specific regions of Gallus gallus GAPDH (NM_204305.1)
6.2	β-Actin-R2	GGAGGGCGTAGCCTTCATAGA
7.1	GAPDH-F	CCAGAACATCATCCCAGCGTC
7.2	GAPDH-R	ACGGCAGGTCAGGTCAACAA