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BioMed Research International
Volume 2017 (2017), Article ID 1080157, 10 pages
https://doi.org/10.1155/2017/1080157
Research Article

MicroRNA Expression Varies according to Glucose Tolerance, Measurement Platform, and Biological Source

1Biomedical Research and Innovation Platform (BRIP), South African Medical Research Council, Tygerberg, South Africa
2Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa
3Department of Psychiatry, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa
4Division of Medical Physiology, Faculty of Health Sciences, Stellenbosch University, Tygerberg 7505, South Africa
5Department of Biochemistry and Microbiology, University of Zululand, Kwa-Dlangezwa 3886, South Africa

Correspondence should be addressed to C. Pheiffer; az.ca.crm@reffiehp.nemrac

Received 25 January 2017; Revised 28 March 2017; Accepted 10 April 2017; Published 26 April 2017

Academic Editor: Lan-Tao Gou

Copyright © 2017 S. Dias et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Dysregulated microRNA (miRNA) expression is observed during type 2 diabetes (T2D), although the consistency of miRNA expression across measurement platform and biological source is uncertain. Here we report miRNA profiling in the whole blood and serum of South African women with different levels of glucose tolerance, using next generation sequencing (NGS) and quantitative real time PCR (qRT-PCR). Whole blood-derived miRNAs from women with newly diagnosed T2D (), impaired glucose tolerance (IGT) (), and normal glucose tolerance (NGT) () were subjected to NGS, whereafter transcript levels of selected miRNAs were quantified in the whole blood and serum of these women using qRT-PCR. Of the five significantly differentially expressed miRNAs identified by NGS, only the directional increase of miR-27b in women with IGT compared to NGT was confirmed in whole blood and serum, using qRT-PCR. Functional enrichment of miR-27b gene targets identified biological pathways associated with glucose transport and insulin regulation. In conclusion, this study showed poor correlation in miRNA expression profiled using NGS and qRT-PCR and in whole blood and serum. The consistent increased expression of miR-27b in women with IGT compared to NGT across measurement platform and biological source holds potential as a biomarker for risk stratification in our population.