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BioMed Research International
Volume 2017 (2017), Article ID 1080157, 10 pages
https://doi.org/10.1155/2017/1080157
Research Article

MicroRNA Expression Varies according to Glucose Tolerance, Measurement Platform, and Biological Source

1Biomedical Research and Innovation Platform (BRIP), South African Medical Research Council, Tygerberg, South Africa
2Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa
3Department of Psychiatry, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa
4Division of Medical Physiology, Faculty of Health Sciences, Stellenbosch University, Tygerberg 7505, South Africa
5Department of Biochemistry and Microbiology, University of Zululand, Kwa-Dlangezwa 3886, South Africa

Correspondence should be addressed to C. Pheiffer

Received 25 January 2017; Revised 28 March 2017; Accepted 10 April 2017; Published 26 April 2017

Academic Editor: Lan-Tao Gou

Copyright © 2017 S. Dias et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Table S1 lists the assay names of differentially expressed miRNAs identified by next generation sequencing, and the mature miRNA sequences that were used to design miRNA-specific stem-loop primers and probes for miRNA profiling with quantitative real time PCR. Supplementary Table S2 lists the GO terms enriched for miR-27b gene targets, which are categorised into 41 molecular functions, 404 biological processes and 11 cellular components. The supplementary table lists: 1) GO term ID and name, 2) the total number of differentially expressed and background population genes associated with each GO term ID, 3) fold enrichment values, 4) p-values, 5) false discovery rate, 6) enrichment scores and 7) gene ratio values. Supplementary Table S3 lists the significantly enriched signalling pathways identified for miR-27b gene targets using KEGG pathway analysis. The table lists: 1) pathway ID, 2) definition of the pathway ID, 3) website for differentially expressed genes, 4) fisher p-value, 5) the total number of differentially expressed and background population genes associated with pathway ID, 6) false discovery rate, 7) enrichment scores and 8) gene ratio values.

  1. Supplementary Material