Antagonistic Properties of Some Halophilic Thermoactinomycetes Isolated from Superficial Sediment of a Solar Saltern and Production of Cyclic Antimicrobial Peptides by the Novel Isolate Paludifilum halophilum

Table 4

Phenotypic characteristics of potential actinomycete isolates.

Characteristics	STS2	STS3	STC4	SMBg3	SMC3	STB8
Pond/growth medium	TS/SCA	TS/SCA	TS/SCA	M1/Bg	M1/CA	TS/Bt
Morphological characteristics
Colony aspect	Circular	Circular	Circular	Circular	Circular	Circular
Colony size (mm)	4–8	4–8	2–4	4–8	4–8	1–5
Aerial mycelium	Absent	Absent	Yellow	Pale yellow	Yellow	Yellow
Substrate mycelium	White	White	Colorless	Yellow pale	Yellow pale	Transparent
Spore chains/fluorescence	Short chains/fluorescent	Long chains/very fluorescent	Short chains/fluorescent	Long chains/fluorescent	Short chains/fluorescent	Long chains/fluorescent
Biochemical characteristics
Gram staining	+	+	+	+	+	+
Catalase	+	+	+	+	+	+
Oxidase
Mannitol	−	−	−	−	−	−
Citrate	−	−	−	−	+	−
H2S production	−	−	−	−	−	−
Nitrate reductase	−	−	+	−	−	+
Nitrite reductase	−	−	−	−	−	−
ONPG	−	−	−	−	−	−
Urea	−	−	−	−	+	+
Indole production	−	−	−	−	+	−
RM	−	−	−	−	−	+
VP	−	−	−	−	−	−
Growth characteristics
% NaCl range (optimum)	5–15 (10)	5–20 (10)	5–20 (10)	5–20 (10)	5–20 (10)	5–15 (10)
Temperature range (optimum)	30–55 (45)	30–55 (45)	30–55 (45)	30–55 (45)	30–55 (45)	30–55 (45)
pH range (optimum)	5–11 (8)	5–11 (8)	5–11 (8)	5–11 (8)	5–11 (8)	5–11 (8)
Carbon source utilization (1%)
Glucose	+	+	+	+	+	+
Sucrose	−	−	−	−	−	−
D(+) Saccharose	+/−	+	+	+	+	+
D Maltose	+/−	+	+	+	+	+
D-Xylose	+/−	+	+	+	+	+
Mannitol	−	+	+	+/−	+	+
Starch	+	+	+	+	+	+