Whole Genome Amplification of Day 3 or Day 5 Human Embryos Biopsies Provides a Suitable DNA Template for PCR-Based Techniques for Genotyping, a Complement of Preimplantation Genetic Testing

<div>GAPDH, SRY, and DYS14 melting curve analysis. Isolated genomic DNA from control adults (Genomic) and WGA from a blastomere were used as templates for GAPDH, SRY, and DYS14 qPCR reactions. Representative data for males and females are shown. (a) Melting temperature peaks were determined by negative first derivatives (−<i>dF/dT</i>) plot. These plots were used for identification of the embryo’s sex. Melt curve data was extracted from the StepOne software and used to create graphs. Representative graphs for each gene and sample type are shown. (b) PCR products were resolved through capillary electrophoresis using the BioAnalyzer Labchip GX. Products showed a single band corresponding to the predicted base pair length.</div>

BioMed Research International

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Figure 1

Figure 1: Whole Genome Amplification of Day 3 or Day 5 Human Embryos Biopsies Provides a Suitable DNA Template for PCR-Based Techniques for Genotyping, a Complement of Preimplantation Genetic Testing 

Figure 1 | Whole Genome Amplification of Day 3 or Day 5 Human Embryos Biopsies Provides a Suitable DNA Template for PCR-Based Techniques for Genotyping, a Complement of Preimplantation Genetic Testing 