Whole Genome Amplification of Day 3 or Day 5 Human Embryos Biopsies Provides a Suitable DNA Template for PCR-Based Techniques for Genotyping, a Complement of Preimplantation Genetic Testing

Figure 2

Capillary electrophoresis analysis of amelogenin intron 1. Male and female adult DNA, isolated from 10–15 cells from a buccal sample, were prepared under the same conditions as DNA isolated from Day 5 embryos. PCR products were separated by capillary electrophoresis to confirm AMELX (104 bp) and AMELY (110 bp) for male (a) and female adults (b). Comparison of the AMELX (c), AMELY (d), and peak height and X/Y ratio (e) for Day 3 and Day 5 embryo biopsies. The height of the bar is the average and the error bars represent the 95% CI. indicates a significant difference between groups ().

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