Whole Genome Amplification of Day 3 or Day 5 Human Embryos Biopsies Provides a Suitable DNA Template for PCR-Based Techniques for Genotyping, a Complement of Preimplantation Genetic Testing

Table 1

qPCR primers.

Gene	Forward primer	Reverse primer	Size	Ref.
GAPDH	5′-TTTAACTCTGGTAAAGTGGATATTG-3′	5′-CTGTTGTCATACTTCTCATGGTT-3′	363 bp	This work
SRY	5′-GCTGGGATACCAGTGGAAAA-3′	5′-TCTTGAGTGTGTGGCTTTCG-3′	247 bp	[22, 23]
DYS14	5′-CATCCAGAGCGTCCCTGG-3′	5′-TTCCCCTTTGTTCCCCAAA-3′	147 bp	[24]
AMEL	5′-CCCTGGGCTCTGTAAAGAATAGTG-3′; (6-carboxyfluorescein labeled)	5′-ATCAGAGCTTAAACTGGGAAGCTG-3′	106 bp (XX); 110 bp (XY)	[25]
TTC3	5′- GAATACTTTGATGATTGCCAACAG-3′	5′-TCACTAGAATACTGCTTCGAGAC-3′	141 bp	This work
RPL17	5′-CCCCACTTAGATGTACATAGCC-3′	5′-TGGAGGACTTCAGCTTATTCTG-3′	236 bp	This work
HSD3B2	5′-CCCACTCCATACCCGTACAG-3′	5′-GTAGAGAACTTTCCAACACTTGAC-3′	206 bp	This work