Whole Genome Amplification of Day 3 or Day 5 Human Embryos Biopsies Provides a Suitable DNA Template for PCR-Based Techniques for Genotyping, a Complement of Preimplantation Genetic Testing

Table 3

Detection for Trisomy 18 and Trisomy 21 for embryo samples.

Gene	Group
TTC3	Normal	21	4.77 ± 0.56	—		—
	Trisomy 18	6	4.81 ± 1.49	0.536	—	0.66 (0.36–0.96),
	Trisomy 21	13	3.39 ± 0.99	<0.001	0.008	0.89 (0.74–1.00),  p < 0.001
RPL-17	Normal	21	2.19 ± 0.80	—		—
	Trisomy 18	9	0.53 ± 0.53	<0.001	—	0.94 (0.86–1.00),  p < 0.001
	Trisomy 21	13	1.77 ± 0.87	0.400	0.002	0.50 (0.32–0.69),

equals the number of embryos per a group. For TTC3, 3 embryos with Trisomy 18 had values greater than 30 and were excluded from the analysis. are mean threshold cycle () ± stand deviation; value for the comparison between Trisomy embryos and normal embryos using ANOVA with a post hoc Bonferroni test; value for the comparison between Trisomy 18 embryos and Trisomy 21 embryos using ANOVA with a post hoc Bonferroni test. area under a Receiver-Operator Characteristic Curve (AUC) between Trisomy groups and the normal group. Values are expressed as AUC (95% confidence interval), value.