A Simple but Accurate Method for Evaluating Drug-Resistance in Infectious HCVcc System

Figure 1

Verification of drug-resistant viruses. Huh7.5 (HCV+) cells were treated with 1 μM of simeprevir ((a), right) or sofosbuvir ((b), right) or solvent control ((a) and (b), left). At 24 hours, the cells were washed and continuously incubated with fresh culture media containing drugs again for 48 hours. The cultural supernatants were then harvested and directly incubated to naïve Huh7.5 cells. After having been passaged 1~3 times, the newly infected cells were treated with 1 μM of simeprevir (a) or sofosbuvir (b) for 72 hours. Intracellular proteins were extracted and detected with WB. and versus solvent control. All of the experiments were performed for over 3 times. The bands presented in the figure were from a representative experiment. Presented is mean ± standard deviation and Student’s t-test was used.

(a)

(b)