A Simple but Accurate Method for Evaluating Drug-Resistance in Infectious HCVcc System

Figure 2

The anti-HCV activity of drugs against WT and mutant HCV in Huh7.5 cells. Naïve Huh7.5 cells were infected with wild and mutant type HCV and simultaneously treated with drugs. Intracellular proteins were extracted and detected with WB in 72 hours. (a) Simeprevir against WT and D168V mutant HCV. ((b)–(f)) Sofosbuvir against WT or H118R (b), E333G (c), N335S (d), E333G + N335S (e), or S282T (f) mutant HCV. (g) Telaprevir (VX-950) against WT and A156T mutant HCV. and versus solvent control. All of the experiments were performed for over 3 times. The bands presented in the figure were from a representative experiment. Presented is mean ± standard deviation and Student’s t-test was used.

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