Research Article

Ethyl Acetate Extracts of Semen Impatientis Inhibit Proliferation and Induce Apoptosis of Human Prostate Cancer Cell Lines through AKT/ERK Pathways

Figure 5

EAESI induces apoptosis in PC3, RV1, and LNCaP cells through AKT ERK pathway. PC3, RV1, and LNCaP cells were treated with different concentrations (0, 40, and 80 μg/ml) of EAEIB for 24 h. The expression levels of BAX, Bcl-2, caspase-3, Akt, phospho-Akt, ERK, and phospho-ERK were determined by Western blot. β-Actin was used as a loading control. The results are representative of 3 independent experiments.