Biochemical and Molecular Analysis of the Hb Lepore Boston Washington in a Syrian Homozygous Child

Figure 4

Multiplex ligation-dependent probe amplification performed using SALSA MLPA Kit P102 HBB. The horizontal axis shows the MLPA probes arranged according to chromosomal location. The vertical axis shows the normalized probe ratio. The complete absence of peaks corresponding to the probes HBDex3-399nt through HBBintr1-154nt indicates a homozygous deletion of the 3′ end of the δ-globin gene and 5′ end of the β-globin gene.