Biochemical and Molecular Analysis of the Hb Lepore Boston Washington in a Syrian Homozygous Child

Table 1

Tabulated details of PCR conditions.

Primer pair	Target region	MgCl2 (mM)	dNTPs (μM)	Thermal
β1-β2	HBB (5′ UTR to exon 2)	3.5	250	-- –
β7-β8	HBB (exon 2 to IVS 2)	3.0	′′	-- –
β9-β10	HBB (IVS 2 to 3′ UTR)	1.5	′′	-- –
-β2	HBD 5′ UTR to HBB; exon 2	′′	′′	-- –

thermal profile was preceded by denaturation at 94°C for 3 min.
  δ1-β2 primer pair is used in GAP-PCR. The distance between the primers is too great (>8 Kb) to amplify the normal allele and product is only obtained from the deleted one where fusion is present.