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BioMed Research International
Volume 2017 (2017), Article ID 1268623, 10 pages
https://doi.org/10.1155/2017/1268623
Research Article

Genetic Variation and Its Reflection on Posttranslational Modifications in Frequency Clock and Mating Type a-1 Proteins in Sordaria fimicola

1Molecular Genetics Research Laboratory, Department of Botany, University of the Punjab, Lahore 54590, Pakistan
2Institute of Industrial Biotechnology, Government College University, Lahore 54590, Pakistan
3Department of Biological Sciences, Macquarie University and CSIRO Black Mountain Laboratories, Building 101, Clunies Ross Street, Black Mountain, ACT 2601, Australia

Correspondence should be addressed to Hamid Mukhtar

Received 12 December 2016; Revised 24 March 2017; Accepted 5 April 2017; Published 21 June 2017

Academic Editor: Vasiliki Galani

Copyright © 2017 Rabia Arif et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Posttranslational modifications (PTMs) occur in all essential proteins taking command of their functions. There are many domains inside proteins where modifications take place on side-chains of amino acids through various enzymes to generate different species of proteins. In this manuscript we have, for the first time, predicted posttranslational modifications of frequency clock and mating type a-1 proteins in Sordaria fimicola collected from different sites to see the effect of environment on proteins or various amino acids pickings and their ultimate impact on consensus sequences present in mating type proteins using bioinformatics tools. Furthermore, we have also measured and walked through genomic DNA of various Sordaria strains to determine genetic diversity by genotyping the short sequence repeats (SSRs) of wild strains of S. fimicola collected from contrasting environments of two opposing slopes (harsh and xeric south facing slope and mild north facing slope) of Evolution Canyon (EC), Israel. Based on the whole genome sequence of S. macrospora, we targeted 20 genomic regions in S. fimicola which contain short sequence repeats (SSRs). Our data revealed genetic variations in strains from south facing slope and these findings assist in the hypothesis that genetic variations caused by stressful environments lead to evolution.