Proliferative and Invasive Effects of Progesterone-Induced Blocking Factor in Human Glioblastoma Cells
PIBF (57 kDa) isoform is regulated by P4 in glioblastoma cells. Western Blot for PIBF protein was performed in U87 cells treated with vehicle (V, cyclodextrin 0.02%), P4 (10 nM), RU486 (10 μM), and P4 plus RU486 (P4 + RU) for 12 and 24 h. Representative images of PIBF isoforms content are shown with their respective densitometric analysis after 12 h (a) and 24 h (b) of treatment. For the densitometric analysis, PIBF values were corrected with those of the internal control, α-tubulin. The data are expressed as the mean ± S.E.M. with ; versus the other treatment groups. (c) PIBF expression was silenced using a specific siRNA and a control siRNA that lacks any known mRNA target sequence. The image shows the reduction of both PIBF isoforms as evaluated by Western Blot.
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