Calcium Ionophore, Calcimycin, Kills Leishmania Promastigotes by Activating Parasite Nitric Oxide Synthase

Figure 3

Blocking calcimycin antipromastigote activity by ARL-17477 and L-NNA, specific inhibitors of constitutive NOS. The metabolic capacity of L. major promastigotes in cultures treated with 0.5 μM calcimycin and/or the specific inhibitors of constitutive NOS ARL-17477 (50 μM) and L-NNA (100 μM) in comparison with untreated culture (taken as 100%). Data are presented as mean ± SD (three independent experiments; each assay was performed in triplicate). Promastigote survival was compared using Mann–Whitney test. corresponds to ; corresponds to .