Overexpression of Ubiquinol-Cytochrome c Reductase Core Protein 1 May Protect H9c2 Cardiac Cells by Binding with Zinc

Figure 2

The effect of the interaction between UQCRC1 overexpression and Zn²⁺ on cardiac cell protection. (a) Viability of H9c2 cardiac cells after OGD/R injury ( versus Ad-GFP, versus Ad-UQCRC1, and versus Ad-UQCRC1; ). (b) Active caspase-3 expression in H9c2 cardiac cells assessed by Western blotting after OGD/R injury ( versus Ad-GFP and versus Ad-UQCRC1; ). (c) Confocal fluorescence images of TMRE (C1: Ad-GFP, C2: Ad-UQCRC1, C3: Ad-UQCRC1+Zn²⁺, and C4: Ad-UQCRC1+TPEN). (d) Mitochondrial membrane potential of H9c2 cells assessed by TMRE after H₂O₂ injury (summarized data for TMRE fluorescence intensity; versus Ad-GFP and versus Ad-UQCRC1; ). Data are expressed as the means ± SEM.

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