An Imaging and Histological Study on Intrahepatic Microvascular Passage of Contrast Materials in Rat Liver

Figure 5

Stepwise hepatic portography and histomorphology from a rat liver. (a) An excised rat liver with the PV catheterized; (b) DR before contrast injection; (c, d, e, f) microangiograms after stepwise infusion of BSS into the PV display a gradual opacification of hepatic vascular tree solely composed of the PV branches with cloudy filling of the adjacent sinusoids but without discernable HA branches; (g, h, i) photomicrographs of a HE-stained paraffin slice focusing on a cross-sectional portal triad demonstrate the full presence of BSS granules in the PV cavity and the surrounding sinusoids but absence in the HA and PVP cavities (original magnifications of 50, 100, and 200); (j, k, l) microscopic HE-stained oblique-sectional views of a portal triad from the same group uncover the BSS particles mainly in the PV cavity and adjacent sinusoids (original magnifications of 100, 200, and 400); (m, n, o) HE-stained photomicrographs of another portal triad oblique section from the same group confirm full presence of BSS granules in the cavities of both the PV and sinusoids but obscurely in the HA and PVP cavities (original magnifications of 50, 100, and 200). Green arrow points bile duct.

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