Table 3: Effect of individual vegetable and blended oil supplementation as carbon source on M. alpina CFR-GV15 on biomass, total lipid, and omega-3 fatty acid production.

Oil supplementDry biomass
(g/L)
Total lipid
(% w/w)
Fatty acid content (% w/w)
18:3 (n-3)20:4 (n-6)20:5 (n-3)22:6 (n-3)

GY medium
MOND
RBOND
SBOND
SFOND
GCO
CO
LSO
Blend oil supplement (2% level)
LSO + SFO
LSO + SBO
LSO + RBO
LSO + MO
LSO + GCO
LSO + CO
GCO + CO

Culture conditions: GY medium: glucose and yeast extract 2 : 1 ratio; pH 6.5; 230 rpm; cultivation period 7-day values of means SD (control). Optimized medium carbon source: 1% carbon and 0.5% yeast extract; 1% KNO3; 0.1% KH2PO4 and MgSO4·7H2O 0.05%; additional 2% individual vegetable and blended oil supplementation; pH 6.5; 230 rpm; cultivation period: 9-day values of means ± SD, n-3. Values in the same column that do not share the same alphabetic superscripts are significantly different at levels according to Duncan’s multiple range tests.