Abdominal Manual Therapy Repairs Interstitial Cells of Cajal and Increases Colonic c-Kit Expression When Treating Bowel Dysfunction after Spinal Cord Injury

Figure 7

Gene and protein expression levels of c-kit in different groups. After therapy, the gene expression levels of c-kit were evaluated by RT-PCR (a), normalized with the internal control GAPDH (). Western blot was used for c-kit protein level assessment, with tubulin as a loading control (). The sham, SCI, SCI + Drug, SCI + LMT, and SCI + HMT groups were analyzed. Representative western blot images are displayed in the bottom panel. , the SCI group compared with the sham group; , compared with the SCI group; or , compared with the SCI + Drug group; , compared with the SCI + LMT group. .

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(b)