Chlamydia muridarum Infection of Macrophages Stimulates IL-1β Secretion and Cell Death via Activation of Caspase-1 in an RIP3-Independent Manner

Figure 7

C. muridarum induced the activation of NLRP3 inflammasome independent of RIP3. WT, RIP3^−/−, RIP3^−/−Caspase-1^−/− BMDMs were infected by C. muridarum (MOI = 10) for 12 h and 24 h. Supernatants were analyzed for the secretion of IL-1β by ELISA (a). Western blot of both supernatants and lysates was performed for the detection of IL-1β (b) and Caspase-1 (c). The figure is shown by combining the values of three independent experiments; values = mean ± SE; .

(a)

(b)

(c)