Prokaryotic Expression of α-13 Giardin Gene and Its Intracellular Localization in Giardia lamblia

Figure 2

SDS-PAGE (a, b) and Western blot (b) analysis of α-13 giardin fusion protein. (a) M, standard protein marker; 1, pET-28a(+)/BL21(DE3); 2, total cell lysate of pET-28a(+)-g-α13 induced with ZYM-5052 medium; 3, total cell lysate of pET-28a(+)-g-α13 induced with 0.5 mM IPTG; 4, total cell lysate of uninduced pET-28a(+)-g-α13; and 5, BL21(DE3). (b) M, standard protein marker; 1, ultrasound supernatant of pET-28a(+)-g-α13/BL21(DE3); and 2, ultrasound precipitation of pET-28a(+)-g-α13/BL21(DE3). (b) 3, total cell lysate of pET-28a(+)-g-α13/BL21(DE3) induced with 0.5 mM IPTG and 4, total cell lysate of pET-28a(+)-g-α13/BL21(DE3).

(a)

(b)