The expression of FGF21 and cell cycle exit genes was increased in the early stage of myogenic differentiation. (a) C2C12 myoblast cells were differentiated for 0 and 2 days, and mRNA levels of FGF21 and myogenic differentiation key genes, MyoG, MyoD, and Myf5, were measured using Q-PCR. (b) The mRNA expression levels of cell cycle inhibitor genes were determined in C2C12 cells after being differentiated for 0 and 2 days by Q-PCR. (c) Western blot was applied to detect the changes in protein level of FGF21, myogenesis genes, and cell cycle related genes. (d) FGF21 protein level in cell medium at 0- and 2-day differentiation was measured by ELISA. GAPDH and tubulin were used as internal control for Q-PCR and western blot, respectively. The expression level of genes at day 0 was set as 1. All the data are presented as the mean ± SD (). ,.
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