Myogenesis was enhanced while cell cycle was suppressed in FGF21 stable expression myoblasts. (a) The mRNA expression levels of myogenic differentiation determination genes were detected in FGF21 stably transfected C2C12 cells. (b) Immunofluorescence of MHC in FGF21 overexpression or control C2C12 cells after 2 and 4 days of differentiation. (c) The protein levels of genes involved in myogenic differentiation as well as cell cycle were determined by western blot in FGF21 overexpression cells. (d) The mRNA levels of cell cycle related genes were determined by Q-PCR in FGF21 overexpression or control C2C12 cells. (e) Quantification of cell cycle distribution of FGF21 stably transfected cells analyzed by flow cytometry. GAPDH and tubulin were used as internal control for Q-PCR and western blot, respectively. For Q-PCR, the expression level of genes in the control group was set as 1. All the data are presented as the mean ± SD (). ,.
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