Dexmedetomidine Protects Cardiomyocytes against Hypoxia/Reoxygenation Injury by Suppressing TLR4-MyD88-NF-κB Signaling

Figure 4

TLR4 knock-down by TLR4 siRNA transfection reduces TLR4 and nuclear NF-kB p65 levels in cardiomyocytes exposed to H/R. IL-1β, TNF-α, and IL-6 expression was measured by quantitative RT-PCR (a–f). Data are expressed as means ± SEM ( = 3 per group). Western blots were used to measure TLR4 and nuclear NF-κB p65 protein expression in both H9C2 cells and neonatal cardiomyocytes (g–j). GAPDH and lamin B were used as a loading control for total and nuclear protein expression, respectively. Data are expressed as means ± SEM ( = 3 per group). < 0.05, < 0.01 versus control group; < 0.05, < 0.01 versus H/R group. D, DEX.

(a) H9C2 cells

(b) Neonatal cardiomyocytes

(c) H9C2 cells

(d) Neonatal cardiomyocytes

(e) H9C2 cells

(f) Neonatal cardiomyocytes

(g) H9C2 cells

(h) Neonatal cardiomyocytes

(i) H9C2 cells

(j) Neonatal cardiomyocytes