Dexmedetomidine Protects Cardiomyocytes against Hypoxia/Reoxygenation Injury by Suppressing TLR4-MyD88-NF-κB Signaling

Figure 6

Overexpression of TLR4 reverses the inhibitory effects of DEX on TLR4, MyD88, and nuclear NF-kB p65 expression. IL-1β, TNF-α, and IL-6 expression was measured by quantitative RT-PCR (a–f). Data are expressed as means ± SEM ( = 3 per group). Western blot analysis was used to measure TLR4, MyD88, and nuclear NF-κB p65 protein expression in both H9C2 cells and neonatal cardiomyocytes (g–l). GAPDH and lamin B were used as a loading control for total and nuclear protein expression, respectively. Data are expressed as means ± SEM ( = 3 per group). < 0.05, < 0.01 versus H/R group; < 0.05, < 0.01 versus D + H/R group. D, DEX.

(a) H9C2 cells

(b) Neonatal cardiomyocytes

(c) H9C2 cells

(d) Neonatal cardiomyocytes

(e) H9C2 cells

(f) Neonatal cardiomyocytes

(g) H9C2 cells

(h) Neonatal cardiomyocytes

(i) H9C2 cells

(j) Neonatal cardiomyocytes

(k) H9C2 cells

(l) Neonatal cardiomyocytes