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BioMed Research International
Volume 2017, Article ID 1762162, 7 pages
https://doi.org/10.1155/2017/1762162
Research Article

Multilocus Sequencing of Corynebacterium pseudotuberculosis Biotype Ovis Strains

1CAR, HAS, Institute for Veterinary Medical Research, P.O. Box 18, Budapest 1581, Hungary
2Government Office for Borsod-Abaúj-Zemplén County, Vologda U. 1, Miskolc 3525, Hungary
3Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, P.O. Box 22, Budapest 1581, Hungary

Correspondence should be addressed to László Makrai; uh.tevinu@olzsal.iarkam

Received 4 March 2017; Revised 26 July 2017; Accepted 8 August 2017; Published 11 October 2017

Academic Editor: Chiu-Chung Young

Copyright © 2017 Boglárka Sellyei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Thirteen Corynebacterium pseudotuberculosis biotype ovis strains isolated from clinical cases of caseous lymphadenitis in Hungary were characterised using multilocus sequencing and their phylogenetic comparison was carried out on the basis of four housekeeping genes (groEL1, B, dnaK, and leuA). The in silico analysis of the 16 frequently studied housekeeping genes showed that C. pseudotuberculosis strains could be readily distinguished from C. diphtheriae and C. ulcerans strains; however, sequences of the same genes in the two biotypes of the C. pseudotuberculosis were highly similar; the heterogeneity values were low. Genes dnaK, B, groEL1, and leuA showed marked genetic variation within C. pseudotuberculosis, and strains of the two biotypes of C. pseudotuberculosis could be differentiated. Analysis of the individual genes showed a fairly conservative nature of C. pseudotuberculosis biotype ovis strains. The greatest genetic differentiation was seen in the dnaK and infB genes and concatenations of these two genes were very useful in the genetic separation of the studied strains.