Multilocus Sequencing of Corynebacterium pseudotuberculosis Biotype Ovis Strains

Table 3

Primer sequences used for amplification and sequence analysis.

Locus	Putative gene product	Primer name	Sequence	Product size (bp)	Position on NC_014329.1 (FRC41 strain)
dnaK	Chaperone Hsp70	Cps dnaK F	5′-TCCTTACCAGTGCCCTTATCC-3′	580	2081944–2081964
		Cps dnaK R	5′-GAGTTCCAGCGCATCACC-3′		2082507–2082524
groEL1	Heat shock protein	Cps groEL1 F	5′-ACCTTCACCGGATCATTG-3′	654	1978987–1979004
		Cps groEL1 R	5′-TTGGTGATCGTCGTAAAGC-3′		1979623–1979641
B	Translation initiation factor IF2	Cps infB F	5′-ATTGCGGGACTTGGACG-3′	641	1397768–1397784
		Cps infB R	5′-GCATTATGCTGCACAAGACG-3′		1398390–1398409
leuA	2-isopropylmalate synthase	Cps leuA F	5′-AGCTCAGTGCGCGGTTGACC-3′	647	161252–161271
		Cps leuA R	5′-ATGGCGTCGCGGGTTCG-3′		161915–161899