Fibrillar Type I Collagen Enhances the Differentiation and Proliferation of Myofibroblasts by Lowering α2β1 Integrin Expression in Cardiac Fibrosis

Figure 3

Knocking down β1 integrin enhanced the differentiation and proliferation in myofibroblasts on FC. (a) Proliferation assay () and (b) the expression levels of α2 and β1 integrin, PTEN, p-AKT, and AKT were analyzed in shRNA-control or shRNA-β1 integrin transfected fibroblasts on FC at day 4 by Western blot. (c) α-SMA expression by Western blots in shRNA-control or shRNA-β1 integrin transfected fibroblasts. (d) The proliferation assay (). (e) The expression levels of α2 and β1 integrin, PTEN, p-AKT, and AKT in shRNA-control or shRNA-PTEN transfected fibroblasts on FC at day 4 by Western blot.

(a)

(b)

(c)

(d)

(e)