Fibrillar Type I Collagen Enhances the Differentiation and Proliferation of Myofibroblasts by Lowering α2β1 Integrin Expression in Cardiac Fibrosis

Figure 5

Low PP2A activity promoted the differentiation and proliferation in cardiac fibroblasts on FC. (a) The proliferation assay of fibroblasts with or without okadaic acid treatment on FC (). (b) The proliferation assay of fibroblasts (). (c) Western blots showed the expressions of PP2Ac, PTEN, AKT, p-AKT, α-SMA, and actin at day 4 in shRNA-control or shRNA-β1 integrin transfected fibroblasts on FC. (d) The expressions of PTEN, α-SMA, actin, p-AKT, AKT, p-ERK, and ERK by Western blot in fibroblasts with or without okadaic acid treatment on FC. (e) Analysis of proliferation (left panel) and of the expressions of p-AKT, AKT, p-ERK, and ERK by Western blot in fibroblasts treated with control, UO126, wortmannin, or UO126 + wortmannin on FC at day 4 (right panel). (f) Analysis of PTEN activity in fibroblasts treated with or without okadaic acid treatment on FC. Representative immunoblots from three independent experiments are shown.

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