Protective Effect of a Polyherbal Aqueous Extract Comprised of Nigella sativa (Seeds), Hemidesmus indicus (Roots), and Smilax glabra (Rhizome) on Bleomycin Induced Cytogenetic Damage in Human Lymphocytes

Table 1

Mean numbers of various chromosomal aberrations after damage induced by bleomycin (40 µg/mL) in the presence or absence of the decoction (100–600 µg/mL). DC, dicentric chromosome; AF, acentric fragments; ChB, chromatid breaks.

Experimental groups	Concentration; (μg/mL)	DC per 100 spreads	DC%; inhibition	AF per; 100; spreads	AF%; inhibition	ChB; per 100; spreads	ChB%; inhibition
Negative control		0.037 ± 0.01		0.043 ± 0.01		0.063 ± 0.01
Decoction control	Dec. 100; 300; 600	0.027 ± 0.01; 0.026 ± 0.01; 0.029 ± 0.02		0.057 ± 0.01; 0.049 ± 0.02; 0.054 ± 0.01		0.043 ± 0.02; 0.041 ± 0.01; 0.047 ± 0.02
Bleomycin control	BLM 40			0.36 ± 0.02		0.46 ± 0.04
Test (decoction + bleomycin)	BLM 40; Dec. 100; 300; 600	0.17 ± 0.04; 0.14 ± 0.03; 0.16 ± 0.01	37.03; 48.15; 40.74	0.23 ± 0.02; 0.19 ± 0.01; 0.21 ± 0.02	36.11; 47.23; 41.67	0.34 ± 0.03; 0.29 ± 0.02; 0.33 ± 0.01	26.1; 36.96; 28.26