BioMed Research International / 2017 / Article / Fig 4

Research Article

Biocompatibility and Effectiveness Evaluation of a New Hemostatic Embolization Agent: Thrombin Loaded Alginate Calcium Microsphere

Figure 4

Cytotoxicity assay of TACMs. (a) Cell viability of HAVECs grown with different concentrations of microsphere extracts and merely culture medium at 24, 48, and 72 h after incubation. Values were represented as mean ± standard error of the mean (). VECs viability was maintained over 90% at predetermined time points. (b) Cell survival rates of VECs contacting with TACMs for 24 h and 48 h (); the control group was VECs with culture medium alone. There was no significant difference between TACM group and control group (). (c) VECs were stained with calcein-AM and PI at 24 h and 48 h, and cells with green and red fluorescence represented the live and dead cells, respectively.
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