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BioMed Research International
Volume 2017, Article ID 1931204, 8 pages
https://doi.org/10.1155/2017/1931204
Research Article

cAMP Response Element Binding Protein Expression in the Hippocampus of Rhesus Macaques with Chronic Ephedrine Addiction

1Department of Radiology, The First Affiliated Hospital of Shantou University Medical College, No. 57 Changping Road, Shantou, Guangdong 515041, China
2Guangdong Key Laboratory of Medical Molecular Imaging, No. 57 Changping Road, Shantou, Guangdong 515041, China
3Department of Linguistics & Languages, Michigan State University, East Lansing, MI 48824, USA
4Department of Pediatric Surgery, The Second Affiliated Hospital of Shantou University Medical College, No. 69 Dongxiabei Road, Shantou, Guangdong 515041, China
5Department of Forensic Medicine, Shantou University Medical College, No. 22 Xinling Road, Shantou, Guangdong 515041, China

Correspondence should be addressed to Shuhua Ma; moc.621@4102amauhuhs

Received 10 July 2017; Accepted 18 September 2017; Published 18 October 2017

Academic Editor: Luca Cucullo

Copyright © 2017 Zongbo Sun et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Drug addiction is classified as a chronic relapse nature brain disease with complicated neurobiology mechanisms. There are an increasing number of researchers that are investigating the possible mechanisms for solving the thorny problem. Methods. The model of chronic addiction of rhesus monkey ephedrine was established, where changes in body weight and behavior were monitored. The expression of cAMP response element binding protein (CREB) in the hippocampus of rhesus monkeys was identified by real-time PCR and Western blot. Results. We were successful in establishing the chronic ephedrine addiction model in the rhesus macaques. They exhibited changes in body weight and behavior. Immunofluorescence showed that CREB was expressed in the nucleus of the hippocampus, and the expression of CREB mRNA and protein in the hippocampus were increased by real-time PCR and Western blot. The CREB positive expression in the hippocampus of the modeling group was significantly higher than in the control group. Conclusions. The changes of body weight and behavior of the rhesus monkeys after ephedrine chronic addiction were significant. The changes of CREB in the hippocampus of rhesus macaques with ephedrine chronic addiction are important molecular mechanisms, and the upregulation of CREB may be involved in the physiological pathology and behavior process in individuals with chronic ephedrine addiction.