MicroRNA-195 Activates Hepatic Stellate Cells In Vitro by Targeting Smad7
MiR-195 and Smad7 expression in DEN-induced hepatic fibrotic tissues and control tissues. (a) Hepatic fibrotic and control tissues were detected by hematoxylin and eosin (H&E) staining (×200) and Masson staining (×100) to assess liver fibrosis. Black arrows indicate hepatic steatosis, necrosis, and inflammatory cell infiltration. (b) The plasma concentrations of ALT and AST in rats. (c) The mRNA levels of α-SMA in rat liver tissues were detected with qRT-PCR. (d) The mRNA levels of Smad7 in rat liver tissues were detected with qRT-PCR. (e) The protein levels of Smad7 in rat liver tissues were detected with Western blotting. (f) The miR-195 expression in hepatic fibrotic tissues was detected with qRT-PCR. Each value represents the mean ± SD of three experiments. versus control.
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