MicroRNA-195 Activates Hepatic Stellate Cells In Vitro by Targeting Smad7

Figure 6

Interaction of miR-195 with the 3′UTR of Smad7 inHEK 293T cells. (a) miRNA binding sites and mutation site in the 3′UTR of Smad7 mRNA based on miRDB. Primer sequence for the PCR of Smad7 3′UTR MRE (WT) and the mutant (Mut). (b) Based on the pairing sites, corresponding luciferase reporter vectors were designed. (c) The graph depicts luciferase activity in cells transfected with Smad7 3′UTR WT or Smad7 3′UTR Mut as well as the miR-195 mimic or miR-195 mimic control. versus miR-195 mimic control.

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