Early Production of the Neutrophil-Derived Lipid Mediators LTB₄ and LXA₄ Is Modulated by Intracellular Infection with Leishmania major

Figure 2

The release of is reduced in Leishmania major-infected PMN. PMN were infected with L. major promastigotes for 300 minutes. During the last 90 min of infection 10 ng/ml GM-CSF was added. L. major-infected and uninfected neutrophils were coincubated with HT-29 cells and stimulated with ionomycin (1.0 μM) for 10 (a) or 360 (b) min. LXA₄ content of the supernatants was determined by ELISA. (a) , (b) , n.d.: not detectable, , and .

(a)

(b)